Devices for cell separation
AssigneeSouthwest Research Institute
InventorsJian LING, Stephen T. WELLINGHOFF, Carlos MartÍn CANTÚ, Angelica FERNANDEZ
Life of this patent
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Abstract
Devices and methods suitable for cell separation. The devices herein include non-random voids interconnected through non-random pores and/or non-random solid geometrical structures optionally connected through solid non-random interconnecting elements. Such devices are preferably suitable for affinity-based cell isolation techniques which rely upon binding interactions.
Claims20
A method to separate one or more targeted cells from a plurality of cells comprising:
- supplying a device comprising biocompatible polymer material having a plurality of solid geometrical structures having outer surfaces, wherein 90% or more of said geometrical structures have a volume (V) that does not vary by more than +/−10.0%;
- providing a surface coating on said outer surface of the geometrical structures wherein said surface coating provides for cell binding;
- passing a plurality of cells in a fluid through said device to provide a fluid output;
- and
- capturing one or more selected cells from said plurality of cells on the coated surface of said device;
- or
- capturing the one or more selected cells from the plurality of cells in said fluid output.
A method to separate one or more targeted cells from a plurality of cells comprising:
- supplying a device comprising biocompatible polymer material having a plurality of solid geometrical structures having outer surfaces, wherein 90% or more of said geometrical structures have a volume (V) that does not vary by more than +/−10.0% including a plurality of solid interconnecting elements between said structures, wherein 90% or more of said solid interconnecting elements define a volume that does not vary by more than +/−10.0% wherein said solid geometrical structures have a diameter D′ in the range of 2.0 μm to 25.0 mm;
- providing a surface coating on said outer surface of the geometrical structures wherein said surface coating provides for cell binding;
- passing a plurality of cells in a fluid through said device to provide a fluid output;
- and
- capturing one or more selected cells from said plurality of cells on the coated surface of said device;
- or
- capturing the one or more selected cells from the plurality of cells in said fluid output.
A device for cell separation comprising:
- solid geometrical structures have outer surfaces wherein 90% or more of said solid geometrical structures have a volume (V) that does not vary by more than +/−10.0%, including a surface coating on said outer surfaces wherein said surface coating provides for cell binding;
- a plurality of solid interconnecting elements between said solid geometrical structures wherein 90% or more of said solid interconnecting elements define a volume that does not vary by more than +/−10.0%;
- and
- an inlet and outlet to allow for inflow and outflow of a fluid containing cells for separation.
Dependencies are inferred from the claim text.