Flow cytometer module and methods of use thereof

A bidirectional cytometry sampling system including:

• a sample holder;
• a flowcell connected to the sample holder;
• a gantry robot including a pipettor configured to pipette a fluid sample into the sample holder;
• and
• a pump system configured to pump one or more fluids in one or more directions through the flowcell;
• wherein the flowcell is fluidically connected to the sample holder and the pump system.

A modular sheathless flow-cytometry system including:

• a sample holder;
• a pipettor configured to pipette a fluid sample into the sample holder;
• a pump system configured to pump one or more fluids in one or more directions through a flowcell, the flowcell being fluidically connected to the sample holder and the pump system;
• one or more focusing optics;
• a collimated laser configured to shine a beam of light through the one or more focusing optics onto the flowcell;
• at least one side scatter detector configured to receive light scattered by one or more particles flowing in the flowcell;
• at least one forward scatter detector configured to receive the light scattered by one or more particles flowing in the flowcell;
• a forward-scatter mask with one or more forward-scatter mask apertures to limit a collection of forward scattered light to a set of defined scatter angles;
• a processor configured to capture data received by the at least one side scatter detector and the data received by the at least one forward scatter detector to determine at least one characteristic of the one or more particles received by the flowcell;
• and
• a side-scatter optical path, the side-scatter optical path including:
• one or more filters;
• an automated system for inserting and removing the one or more filters;
• and
• a controller configured to operate the automated system.