Nucleic acid construct comprising utr and use thereof

An RNA molecule, comprising:

• (a) an open reading frame (ORF), and
• (b) an untranslated region (UTR), the UTR comprising the following sequence:
• any one of SEQ ID NOs:
• 69 and 83 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 58 and 72 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 59-60 and 73-74 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 61-62 and 75-76 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 63-65 and 77-79 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 66-68 and 80-82 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 70 and 84 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 71 and 85 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 86 and 87 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 92-94 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 99 and 100 or a sequence having at least 90% identity thereto,
• any one of SEQ ID NOs:
• 103 and 104 or a sequence having at least 90% identity thereto, or
• any one of SEQ ID NOs:
• 88-91, 95-98, and 101-102 or a sequence having at least 90% identity thereto.

An isolated polynucleotide, comprising (a) an open reading frame (ORF), the ORF comprising the sequence set forth in any one of SEQ ID NOs:

• 111-113 and 129-131 or a sequence having at least 90% identity thereto,
• wherein optionally, the polynucleotide comprises any one of the following (b)-(d) or any combination thereof:
• (b) a 5′UTR and/or a 3′UTR,
• (c) a poly-A tail, and
• (d) a 5′Cap;
• preferably, the poly-A tail is selected from the group consisting of A120, A30L70, HGH polyA, SV40 polyA, BGH polyA, rbGlob polyA, and SV40late polyA;
• more preferably, the poly-A tail is A120 or A30L70;
• preferably, the 5′Cap is selected from the group consisting of ARCA, 3′-O-Me-m7G(5′)ppp(5′)G, m7G(5′)ppp(5′)(2′OMeA)pU, m7Gppp(A2′O-MOE)pG, m7G(5′)ppp(5′)(2′OMeA)pG, m7G(5′)ppp(5′)(2′OMeG)pG, m7(3′OMeG)(5′)ppp(5′)(2′OMeG)pG, and m7(3′OMeG)(5′)ppp(5′)(2′OMeA)pG;
• preferably, the polynucleotide comprises one or more modifications selected from the group consisting of backbone modifications, sugar modifications, base modifications, and/or lipid modifications, wherein the base modifications are preferably pseudouridine modifications.