Method for Producing a Three-Dimensional Human Multiple-Myeloma Model
AssigneesEtablissement Francais Du Sang, Institut National de la Sante et de la Recherche Medicale, Universite Toulouse III - Paul Sabatier, Centre National De La Recherche Scientifique, Centre Hospitalier Universitaire De Toulouse
InventorsNicolas Lucien ESPAGNOLLE, Mélanie Alexandra GADELORGE, Jill CORRE
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Abstract
The present invention relates to the method for producing a three-dimensional (3D) model of multiple myeloma (MM), in the form of spheroids, by co-culturing mesenchymal stem/stromal cells, endothelial progenitors and primary plasma cells of patient(s) affected by MM. The present invention further relates to the spheroids obtained by said method and the uses thereof.
Claims15
A method for producing human multiple myeloma (MM) spheroids comprising:
- a. Culturing mesenchymal stem/stromal cells (MSCs), endothelial cells and endothelial progenitors in a culture medium;
- b. Harvesting cultured MSCs, endothelial cells and endothelial progenitors;
- and
- c. Co-culturing MSCs, endothelial cells and endothelial progenitors harvested with CD138+ primary plasma cells from a patient with MM under conditions that lead to spheroid formation.
Dependencies are inferred from the claim text.