Thermal hydrolysis of microalgae species for the production of bio-crude and other bioactive molecules

A thermal hydrolysis system of microalgae biomass for producing biocrude and bioactive substances, comprising:

• a high-pressure vessel;
• a vacuum filtration;
• and
• a rotary evaporator.

A method for producing biocrude and bioactive substances from microalgae biomass, comprising the steps of:

• mixing and stirring dry microalgae biomass with water and an acid catalyst;
• processing the biomass mixture in a colloidal mill with solvent;
• separating lysed algae cells into non-polar extracts from water-soluble extracts via vacuum filtration;
• washing the lysed algae cells and non-polar extracts with organic solvents;
• separating the organic solvent mixture, which comprises ethanol and long-chain fatty acids, from the remaining biochar via vacuum filtration;
• and
• separating the ethanol from the fatty acids via distillation or rotary evaporator to extract bio-oil.

A system of microalgae biomass for producing biocrude and bioactive substances, comprising:

• a colloidal mill;
• a vacuum filtration;
• a rotary evaporator;
• and
• a storage tank.