Use of monolithic anion exchange chromatography and light scattering for quantifying extracellular vesicles
AssigneeLonza Sales AG
InventorGang Gary HAO
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Abstract
The present disclosure relates to methods of detecting and purifying EVs (e.g., exosomes) present within a sample using monolithic anionic exchange chromatography and light scattering. Such methods allow for a more accurate and high-throughput method of quantifying EVs present in a sample. Some aspects of the methods provided herein further comprise analyzing one or more additional properties of EVs.
Claims63
A method of determining the amount of extracellular vesicle (EV) present in a sample, the method comprising:
- (i) contacting the sample with a monolithic anion exchange (AEX) chromatography column, and (ii) then measuring a light scattering emission signal from an eluent collected from the AEX chromatography column.
A method of preparing an extracellular vesicle (EV) fraction from a sample, the method comprising:
- (i) contacting the sample with a monolithic anion exchange (AEX) chromatography column, (ii) collecting an eluent from the monolithic AEX chromatography column, and (iii) then measuring a light scattering emission signal from the eluent to prepare the EV fraction.
A method of reducing the amount of impurity present in a sample comprising an extracellular vesicle (EV), the method comprising (i) contacting the sample with a monolithic anion exchange (AEX) chromatography column, (ii) collecting an eluent from the monolithic AEX chromatography column, and (iii) then measuring a light scattering emission signal from the eluent wherein the amount of impurity is reduced in the sample.
Dependencies are inferred from the claim text.