Frozen Biofluids Mitochondrial Bioenergetics Function Assessment Method for Traumatic Brain Injury and Polytrauma

Abstract

A method of assessing mitochondria bioenergetics includes obtaining a frozen tissue or frozen biofluid sample of an individual and measuring mitochondria bioenergetics of the frozen tissue or frozen biofluid sample using a substrate for the frozen tissue or biofluid sample. The substrate may include at least one of Glucose; Alamethicin, Nicotinamide adenine dinucleotide (H), and Cytochrome C; Succinate and Rotenone; or Ascorbate+TMPD (Tetramethyl phenylenediamine).

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