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US 2026/0116943 A1Published application
Processes for synthesizing glucagon-like-peptide 2 (glp-2) analogues
AssigneeZealand Pharma A/S
InventorsClaes MELANDER, Leila MALIK, Jan PAWLAS, Stefan HANSEN
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Abstract
The present invention relates to processes for obtaining glucagon-like-peptide-2 (GLP-2) analogues, such as glepaglutide. In particular, the processes described herein use a multi-step purification method of GLP-2 analogues synthesized by solid phase peptide synthesis (SPPS).
Claims15
A method of producing a GLP-2 analogue synthesized by solid phase peptide synthesis (SPPS), wherein the GLP-2 analogue is represented by the formula:
- wherein:
- R1 is hydrogen, C1-4 alkyl (e.g. methyl), acetyl, formyl, benzoyl or trifluoroacetyl;
- X5 is Ser or Thr;
- X11 is Ala or Ser;
- R2 is NH2 or OH;
- and
- Z2 is absent or a peptide sequence of 1-6 amino acid units of Lys;
- or a pharmaceutically acceptable salt thereof;
- the method comprising the steps of:
- i) loading a column with the crude GLP-2 analogue;
- ii) adjusting the pH of the column with a first buffer system;
- wherein step ii) includes passing the isoelectric point of the peptide while the peptide is on the column by increasing the pH of the column from an acidic pH to a neutral or slightly basic pH.
9 more elements
Dependencies are inferred from the claim text.