Fluidic cartridge with a fluidic chamber

A method of transferring liquid into a fluidic chamber of a fluidic cartridge, the fluidic cartridge comprising;

• a fluidic chamber including
• a first fluidic compartment, and
• a semipermeable membrane,
• wherein the semipermeable membrane separates the first fluidic compartment from a second fluidic compartment of the fluidic chamber,
• wherein the semipermeable membrane is non-permeable for liquid and permeable for air, and
• wherein the second fluidic compartment is a closed compartment configured for accumulating air,
• a pumping device configured for pumping a liquid into the first fluidic compartment such that the first fluidic compartment is filled with the liquid, whereby air contained in the first fluidic compartment is forced through the semipermeable membrane into the second fluidic compartment and accumulates in the second fluidic compartment such that a pressure within the second fluidic compartment increases, and
• a fluidic discharge line coupled to the first fluidic compartment and configured for discharging the first fluidic compartment using the pressure within the second fluidic compartment,
• wherein the method comprises the step of:
• operating the pumping device such that the first fluidic compartment is filled with a liquid, whereby air contained in the first fluidic compartment is forced through the semipermeable membrane into the second fluidic compartment.