Methods for modulating temporal infrastructure of pulsed electric fields

A method of applying electrical energy to tissue, the method comprising:

• administering to a tissue a series of bursts of bipolar electrical pulses, wherein each bipolar electrical pulse comprises a positive phase and a negative phase, wherein each phase has a duration of 250 ns to 250 μs, with or without a delay between the positive and negative phases;
• wherein each bipolar electrical pulse is separated by a delay lasting up to 1 ms;
• wherein the bipolar electrical pulses are repeated until a total energized time of 1 μs to 500 μs is achieved;
• wherein one or more of the bursts has a burst width of at least 1 μs;
• wherein the series of bursts is administered for a total time of 250 ms to 10 seconds;
• and
• wherein the bipolar electrical pulses are applied at a voltage ranging from 500 V to 10 kV.