Systems and methods for wearable sensing platform

A non-invasive wearable device comprising:

• a biofluid generation unit configured to generate, or stimulate the generation of, a biofluid, wherein the biofluid generation unit comprises at least one sweat generating hydrogel, and a muscarinic receptor agonist;
• a microfluidic channel configured to transport the biofluid, wherein the microfluidic channel comprises an input end configured to receive the biofluid and an output end;
• a sensing module in fluid communication with the output end of the microfluidic channel and comprising one or more electrochemical aptamer-based sensors, wherein the one or more electrochemical aptamer-based sensors are functionalized with aptamers comprising attached reporters that are displaceable by a target analyte;
• electrodes configured to produce a potential sufficient for exchanging charged particles with the displaced reporters, or with conjugates of the displaced reporters, wherein the displaced reporters are configured to be hybridized with an enhancer to amplify a signal generated by the displaced reporters;
• and
• a processor communicatively coupled to the sensing module, the biofluid generation unit, the electrode, and a memory configured to store computer readable instructions, wherein the processor is configured to execute the computer readable instructions comprising:
• causing activation of the electrodes to exchange the charged particles with the displaced reporters, or with conjugates of the displaced reporters,
• receiving a measurement of the exchanged charged particles,
• determining a concentration of the target analyte in the biofluid based on the received measurement,
• detecting a circadian rhythm based on the determined concentration;
• and
• generating an individualized set of circadian entraining factors based on the detected circadian rhythm to guide a user towards an improved circadian rhythm.