Back to results
US 2026/0098293 A1Published application
Ssb method
AssigneeOxford Nanopore Technologies PLC
InventorsJames White, Ruth Moysey, Mihaela Misca
Life of this patent
Estimating the term…
Abstract
The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.
Claims13
-38. (canceled)
A method of unblocking a transmembrane protein pore, the method comprising:
- (i) contacting the transmembrane protein pore with a polypeptide under an applied electrical potential;
- (ii) measuring a current through the transmembrane protein pore indicative of blocking of the transmembrane protein pore by the polypeptide;
- and
- (iii) reversing the applied electrical potential to clear the polypeptide from the transmembrane protein pore, thereby unblocking the transmembrane protein pore.
1 more element
Dependencies are inferred from the claim text.