Lysis vessel for on-board lysis

A lysis vessel for performing cell lysis, comprising:

• a laterally extending member having a vent extending therethrough;
• a sleeve depending from the laterally extending member, wherein a bottom end of the sleeve defines an open bottom end of the vessel;
• a first porous membrane affixed to a top or bottom surface of the laterally extending member, the first porous membrane covering the vent, a second porous membrane affixed to the bottom end of the sleeve, the second porous membrane covering the open bottom end, wherein the bottom surface of the laterally extending member, an inner surface of the sleeve and the first and second porous membranes define a lysis chamber;
• a plurality of non-magnetic beads contained within the lysis chamber;
• and
• at least one magnetic element contained within the lysis chamber, wherein the first and the second porous membranes are sized to retain the plurality of non-magnetic beads and the at least one magnetic element within the lysis chamber.