Purification of circular dnas from cells using nanoclays

Abstract

Disclosed herein is a method of purifying circular DNA from a sample by exposure of the sample to a nanoclay that preferentially adsorbs multiple contaminants, including non-circular DNA, RNA, proteins, and endotoxins, while leaving circular DNA substantially unbound in solution. The elution-free method also includes a step of separating nanoclay-contaminant complexes from the enriched circular DNA fraction. Also disclosed herein are compositions and systems operable for purifying circular DNA from a sample. Also disclosed herein are methods of refining, formulating or modifying a nanoclay for use in purifying circular DNA from a sample.

Dependencies are inferred from the claim text.