Development of solid-state storage of complex biochemical systems
AssigneeCal Poly Corporation
InventorJavin Oza
Life of this patent
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Abstract
Compositions for solid-state storage of a biological system and methods of making and using the same are described. The compositions comprise a polymeric cryoprotectant, a saccharide cryoprotectant, an emulsifier, and at least one lubricating flow agent, and are combined with a biological system to store the biological system in a solid-state with or without cold-chain infrastructure. The stabilized biological systems are suitable for use with one or more analytical devices to provide a cold chain-free, point-of-care, and low-cost platform for diagnostics, research, biomanufacturing, and testing.
Claims20
A composition for solid-state storage comprising:
- a polymeric cryoprotectant;
- a saccharide cryoprotectant;
- an emulsifier;
- and
- a lubricating flow agent.
A method of using a stabilized biological system comprising:
- (i) obtaining a stabilized biological system comprising a biological system, a polymeric cryoprotectant, a saccharide cryoprotectant, an emulsifier, and a lubricating flow agent;
- wherein the stabilized biological system is a solid;
- (ii) optionally depositing the stabilized biological system onto a substrate;
- and
- (iii) contacting the stabilized biological system with a liquid to form a resuspension solution.
A paper-based microfluidic device comprising:
- a porous substrate comprising paper;
- an input channel;
- a reaction chamber;
- one or more microfluidic channels;
- and
- a non-porous barrier that defines one or more boundaries for the reaction chamber and the one or more microfluidic channels;
- wherein the reaction chamber is fluidly connected to the input chamber by the one or more microfluidic channels;
- and
- wherein a surface of the microfluidic device is laminated except for the reaction chamber.
Dependencies are inferred from the claim text.