Cell culture electrification

A three-dimensional cell culture device configured to cultivate cells presenting an electric activity, said culture device comprising:

• a plate comprising at least one well extending parallel to a stacking axis (X) and presenting two open extremities, said well being configured to receive at least one cell to be cultivated,
• a permeable bottom sheet configured to retain the at least one cultivated cell while allowing liquid culture medium to flow through and connected to the plate, said bottom sheet closing one open extremity of the well,
• at least one electrode for measuring the electric activity of the cultivated cells,
• at least one connection system configured to connect the at least one electrode to an external acquisition system,
• wherein the cell culture device further comprises an electrode support element connected to the plate, configured to carry the at least one electrode and to enable said at least one electrode to extend inside the well, said electrode being thus configured to extend inside the well,
• wherein the connection system is integrated in the plate.