Method for preparing high-purity and high-stability protein

A method for preparing high-purity and high-stability protein, comprising the following steps:

• step A:
• using a 10 KDa membrane to replace a buffer solution of a recombinant human albumin sample collected from a previous purification, with an equilibrium solution system of 80 mM PB of pH 7.5 to obtain a sample collection solution, wherein a mixed solution is added into the sample collection solution, and the mixed solution contains a mixture of fatty acids and poloxamer, and a molar ratio of oleic acid, myristic acid, sodium palmitate, sodium stearate to recombinant human albumin is 0.3:0.3:0.3:0.5:1, and a weight ratio of the poloxamer to the recombinant human albumin is 10-500 micrograms of the poloxamer per gram of the recombinant human albumin, and the poloxamer is used to promote a dissolution of the fatty acids;
• and
• after sample loading, washing an anion exchange chromatography column, which has a column height of 380 mm, with purified water to neutral, and using an eluent system of a 50 mM PB pH 6.5 to elute an adsorbate in the chromatography column to collect a recombinant human albumin component;
• and
• step B:
• using a 40 mM HAc-NaOH equilibration liquid system to equilibrate a cation exchange chromatography column to collect the recombinant human albumin component, and using a 10 KDa flat sheet membrane to perform desalting and liquid replacement to the collected recombinant human albumin component with a cation exchange chromatography equilibrium liquid, and performing sample loading and chromatography separation to collect the recombinant human albumin component.