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US 2026/0098069 A1Published application
Production of recombinant lubricin
AssigneeLubris LLC
InventorsTannin A. Schmidt, Gregory D. Jay
Life of this patent
Estimating the term…
Abstract
Disclosed are new recombinant isoforms of human-like lubricin or PRG4 glycoprotein having outstanding lubrication properties and a novel glycosylation pattern, and methods for their manufacture at high levels enabling commercial production.
Claims22
1-21. (canceled)
A composition comprising:
- a recombinant multimeric lubricin glycoprotein expressed from a human PRG4 gene in a host cell culture, comprising at least 30% by weight glycosidic residues, and producing a dynamic coefficient of friction no greater than 150% of the dynamic coefficient of friction of purified native bovine lubricin as measured in a cartilage on cartilage friction test,
- wherein at least 90% by weight of glycosylation of the lubricin glycoprotein is core 1 glycosylation, and
- wherein the glycoprotein comprises the amino acid sequence of residues 25-1404 of SEQ ID NO:
- 1.
1 more element
33-40. (canceled)
A recombinant lubricin glycoprotein comprising the amino acid sequence of residues 25-1404 of SEQ ID NO:1, where the glycoprotein comprises at least 90% core 1 glycosylation.
Dependencies are inferred from the claim text.