Fluorescent probe compounds for tumor targeting imaging, and synthesis method therefor

Abstract

The present invention relates to fluorescent probe compounds for tumor-targeted imaging, and methods of synthesis and use thereof. The compounds utilize, for the first time, an antitumor drug comprising a pyrrolo[2,3-d]pyrimidine core structure as a targeting ligand, which is conjugated to a fluorescent dye through a linker moiety. The structure of the compound is shown in Formula I. The compound demonstrates high affinity and selectivity for tumor cells expressing target receptors, enabling localization, qualitative and quantitative analysis through in vitro and in vivo tracking, receptor affinity studies, and mechanism of action investigations, thereby exhibiting high specificity, sensitivity, and visualization capability. The fluorescent probe of the present invention achieves rapid clearance from normal tissues while maintaining prolonged retention at tumor sites, thereby enabling in vivo diagnostic functionality. It demonstrates substantial clinical application potential for clinical intraoperative navigation.

Dependencies are inferred from the claim text.