Epigenetic editing tool for targeting hepatitis b virus gene

A composition comprising:

• (1) a fusion molecule or a nucleic acid sequence encoding the fusion molecule, wherein the fusion molecule comprises at least one DNA binding protein and at least one gene expression modulator, and
• (2) at least one single guide RNA (sgRNA), or a nucleic acid sequence encoding the sgRNA, wherein the sgRNA is complementary to a target DNA sequence in a vicinity of a hepatitis B virus (HBV) gene or within a HBV gene regulatory element, the HBV gene is a type B HBV gene comprising a nucleotide sequence as set forth in SEQ ID NO:
• 1, a type C HBV gene comprising a nucleotide sequence as set forth in SEQ ID NO:
• 2, or a type D HBV gene comprising a nucleotide sequence as set forth in SEQ ID NO:
• 3, the HBV gene regulatory element comprises a transcription initiation site, a core promoter, a promoter, an enhancer, a silencer, an insulator element, a boundary element, or a locus control region, and the target DNA sequence is located between nucleotide at position 1056 and nucleotide at position 2354, between nucleotide at position 2639 and nucleotide at position 2658, between nucleotide at position 2863 and nucleotide at position 2930, or between nucleotide at position 3048 and nucleotide at position 3067 of the HBV gene.

A single guide RNA (sgRNA), wherein the sgRNA comprises a sequence complementary to a target DNA sequence, and the target DNA sequence is located in a vicinity of a hepatitis B virus (HBV) gene or within an HBV gene regulatory element;

• the HBV gene is a type B HBV gene comprising a nucleotide sequence as set forth in SEQ ID NO:
• 1, a type C HBV gene comprising a nucleotide sequence as set forth in SEQ ID NO:
• 2, or a type D HBV gene comprising a nucleotide sequence as set forth in SEQ ID NO:
• 3;
• the HBV gene regulatory element comprises a transcription initiation site, a core promoter, a promoter, an enhancer, a silencer, an insulator element, a boundary element, or a locus control region, and the target DNA sequence is located between nucleotide at position 1056 and nucleotide at position 2354, between nucleotide at position 2639 and nucleotide at position 2658, between nucleotide at position 2863 and nucleotide at position 2930, or between nucleotide at position 3048 and nucleotide at position 3067 of the HBV gene.