Methods and compositions for deprotection of reversibly terminated nucleotides

Abstract

Methods, systems, and kits for sequencing a template nucleic acid molecule using a catalyst-scaffold complex are provided. In some aspects, the catalyst-scaffold complex comprises a palladium catalyst that deblocks allyl groups on reversibly terminated nucleotides for use in a sequencing reaction, such as an in situ sequencing reaction in a sample comprising cells and/or cell nuclei. In some aspects, nucleic acid molecules such as rolling circle amplification products in a cell or tissue sample are sequenced in situ using a catalyst-scaffold complex disclosed herein for deprotecting reversibly terminated nucleotides.

Dependencies are inferred from the claim text.