Methods, systems and devices for screening compounds for efficacy against biofilm formation

A minimum biofilm eradication concentration (MBEC) assay kit, comprising:

• at least one peg assembly comprised of a material of interest;
• and
• at least one well plate and/or trough plate;
• wherein the material of interest comprises metallic materials and/or alloys thereof, stainless steel, nickel, aluminum, titanium, concrete, ceramics, polymers, or a combination thereof.

A method for screening compounds for efficacy against biofilm formation, the method comprising:

• i. providing a peg assembly comprised of a material of interest;
• ii. growing biofilm on pegs of the peg assembly;
• iii. placing the peg assembly onto a challenge plate, wherein the challenge plate comprises serial dilutions of the at least one compound to be screened;
• and
• iv. evaluating the biofilm remaining on the peg assembly.