Bleomycin producing strain and application thereof

Abstract

A production strain of bleomycin and its applications are provided. Through mutagenesis, a strain, Streptomyces mobaraensis BL-G2-202402, which exhibits high resistance to bleomycin, is obtained. Its deposit number is CCTCC NO: M 20241482. By adding bleomycin-backbone amino acids to its fermentation medium formula, the fermentation titer of bleomycin was increased, and the ratio of the main components bleomycin A2 and B2 was affected. When adding 0.5 g/L of bromogeramine and 2.5-10 g/L of oxygen-enhancing agent, the yield of bleomycin could be significantly increased. When using dried bacterial residue to replace part of the nitrogen source in the medium, it was found that fermentation titer increased if freeze-dried bacterial residue replace 10-40% of the corn steep powder as the nitrogen source for bleomycin fermentation.

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