Back to results
US 2026/0092294 A1Published application
Methods of immortalizing primary resting t cells by crispr/dcas9-based epigenetic modifiers and transcriptional activators
AssigneeCity University of Hong Kong
InventorsRaymond Hiu-Wai LAM, Chung TIN, Siping HUANG, Cia Hin LAU
Life of this patent
Estimating the term…
Abstract
A method for extending the replicative lifespan of T cells by activating the TERT gene is provided. Particularly, the method involves the epigenetic modification of the TERT promoter using a CRISPR/dCas9-based epigenetic modifier, followed by the reactivation of the modified TERT promoter using a Cas9-mediated transcriptional activator.
Claims16
A method of extending the replicative lifespan of a T cell by activating the TERT gene, comprising:
- epigenetically modifying a TERT promoter of a T cell using a CRISPR/dCas9-based epigenetic modifier;
- and
- reactivating the modified TERT promoter using a Cas9-mediated transcriptional activator to obtain an immortalized T cell.
A kit for extending a replicative lifespan of a T cell to obtain an immortalized T cell, comprising:
- a CRISPR/dCas9-based epigenetic modifier;
- and
- a Cas9-mediated transcriptional activator.
Dependencies are inferred from the claim text.