Inbred transgenic canola line ns-b50027-4 and seeds thereof

A method of detecting the presence of event NS-B50027-4, said NS-B50027-4 having been deposited under ATCC Accession Number PTA-123186, in a sample comprising plant DNA, wherein said method is a competitive allele-specific PCR-based genotyping assay, and wherein said method comprises:

• contacting said sample with:
• (a) a primer set diagnostic of a transgene-flanking junction region of the Brassica genome and the NS-B50027-4 A05 transgenic cassette selected from the group of primer sets consisting of those depicted in SEQ ID NO:42-SEQ ID NO:44, SEQ ID NO:45-SEQ ID NO:47, SEQ ID NO:48-SEQ ID NO:51, SEQ ID NO:52-SEQ ID NO:55, SEQ ID NO:56-SEQ ID NO:59, SEQ ID NO:60-SEQ ID NO:62, SEQ ID NO:63-SEQ ID NO:65, SEQ ID NO:66-SEQ ID NO:68, SEQ ID NO:69-SEQ ID NO:71, SEQ ID NO:72-SEQ ID NO:74, SEQ ID NO:75-SEQ ID NO:
• 78, SEQ ID NO:
• 79-SEQ ID NO:82, and SEQ ID NO:87-SEQ ID NO:90, and/or
• (b) a primer set diagnostic of a transgene-flanking junction region of the Brassica genome and the NS-B50027-4 A02 transgenic cassette selected from the group of primer sets consisting of those depicted in SEQ ID NO:17-SEQ ID NO:19, SEQ ID NO:20-SEQ ID NO:22, SEQ ID NO:23-SEQ ID NO:25, SEQ ID NO:26-SEQ ID NO:28. SEQ ID NO:29-SEQ ID NO:31, SEQ ID NO:32-SEQ ID NO:34, SEQ ID NO:35-SEQ ID NO:38, SEQ ID NO:39-SEQ ID NO:41, and SEQ ID NO:
• 83-SEQ ID NO:86;
• subjecting said sample to polymerase chain reaction;
• and
• assaying for amplicons generated between said primers.