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US 2026/0092285 A1Published application
Insect resistant inir6 transgenic maize plants lacking a selectable marker and junction
AssigneeInari Agriculture Technology, Inc.
InventorsMichael Lee Nuccio, Joshua L. Price, Michael Andreas Kock
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Abstract
Transgenic INIR6 maize plants comprising modifications of the DP-4114 maize locus which provide for facile excision of the modified DP-4114 transgenic locus or portions thereof, methods of making such plants, and use of such plants to facilitate breeding are disclosed.
Claims21
1-22. (canceled)
23:
- A transgenic maize plant cell comprising a modified transgenic locus comprising a deletion of one or more elements of an original transgenic locus;
- wherein the deletion comprises a deletion of part or all of a phosphinothricin acetyltransferase (PAT) selectable marker cassette and a 3′ junction polynucleotide.
24:
- The transgenic maize plant cell of claim 23, wherein the original transgenic locus is a DP-4114 transgenic locus set forth in SEQ ID NO:
- 1.
25:
- The transgenic maize plant cell of claim 23, wherein the deletion comprises at least the region corresponding to nucleotides 12,734 to 14,370 of SEQ ID NO:
- 1;
- or part of the region corresponding to nucleotides 12,734 to 14,370 of SEQ ID NO:
- 1.
1 more element
26:
- The transgenic maize plant cell of claim 23, wherein the modified transgenic locus comprises SEQ ID NO:
- 25 or an allelic variant thereof.
27:
- The transgenic maize plant cell of claim 23, wherein the deletion comprises a deletion of all of the phosphinothricin acetyltransferase (PAT) selectable marker cassette and part of the 3′ junction polynucleotide.
28:
- The transgenic maize plant cell of claim 27, wherein the deletion of part of the 3′ junction polynucleotide comprises between about 8 to about 1000 base pairs of endogenous chromosomal DNA of the plant genome.
29:
- The transgenic maize plant cell of claim 28, wherein the deletion of part of the 3′ junction polynucleotide comprises between about 10 to 100 base pairs of endogenous chromosomal DNA of the plant genome.
30:
- A transgenic maize plant part comprising the maize plant cell of claim 23, wherein the maize plant part optionally comprises a seed.
31:
- A transgenic maize plant comprising the maize plant cell of claim 23.
32:
- A DNA molecule comprising a modified transgenic locus comprising a deletion of one or more elements of an original transgenic locus;
- wherein the deletion comprises a deletion of part or all of a phosphinothricin acetyltransferase (PAT) selectable marker cassette and a 3′ junction polynucleotide.
33:
- The DNA molecule of claim 32, wherein the original modified transgenic locus is a DP-4114 transgenic locus set forth in SEQ ID NO:
- 1.
34:
- The DNA molecule of claim 32, wherein the deletion comprises a deletion of all of the phosphinothricin acetyltransferase (PAT) selectable marker cassette and part of the 3′ junction polynucleotide.
35:
- The DNA molecule of claim 34, wherein the deletion of part of the 3′ junction polynucleotide comprises between about 8 to about 1000 base pairs of endogenous chromosomal DNA of the plant genome.
36:
- The DNA molecule of claim 32, wherein the deletion of part of the 3′ junction polynucleotide comprises between about 10 to 100 base pairs of endogenous chromosomal DNA of the plant genome.
37:
- A processed transgenic maize plant product comprising the DNA molecule of claim 32.
38:
- A biological sample containing the DNA molecule of claim 32.
39:
- A nucleic acid molecule adapted for detection of genomic DNA comprising the DNA molecule of claim 32, wherein said nucleic acid molecule optionally comprises a detectable label.
40:
- A processed transgenic maize plant product comprising:
- a DNA molecule comprising a modified transgenic locus comprising a deletion of one or more elements of an original transgenic locus;
- wherein the deletion comprises a deletion of part or all of a phosphinothricin acetyltransferase (PAT) selectable marker cassette and a 3′ junction polynucleotide;
- or
- transgenic maize plant cell comprising a modified transgenic locus comprising a deletion of one or more elements of an original transgenic locus;
- wherein the deletion comprises a deletion of part or all of a phosphinothricin acetyltransferase (PAT) selectable marker cassette and a 3′ junction polynucleotide.
3 more elements
41:
- A method of detecting the DNA molecule of claim 40 or a DNA molecule in the transgenic maize plant cell of claim 40, comprising the step of detecting a DNA molecule comprising SEQ ID NO:
- 25 or an allelic variant thereof.
42:
- The method of claim 40, wherein the method comprises detecting a unique transgenic locus excision site in the processed transgenic maize plant product.