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US 2026/0092284 A1Published application
Adeno-associated virus (aav) producer cell lines
AssigneeLonza Houston, Inc.
InventorsBingnan GU, Gang LI, Caitlin TRIPP
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Abstract
The present disclosure relates to nucleic acids encoding helper genes and adeno-associated virus (AAV) genes, under the control of inducible promoters. The disclosure also relates to a mammalian cell line for producing AAV as well as methods of using the mammalian cells for producing AAVs.
Claims21
An isolated nucleic acid molecule operably comprising:
- a. a first inducible promoter and a TetO2 sequence;
- b. an E2A gene under control of the first inducible promoter;
- c. a viral associated (VA) non-coding RNA under control of a second inducible promoter;
- d. a third inducible promoter and a TetO2 sequence;
- e. an E4 gene under control of the third inducible promoter;
- f. an expression cassette encoding a protein ortholog of the VA RNA and a peptide protein tag under the control of a depressible promoter comprising a human Cytomegalovirus (CMV) promoter and a TetO2 sequence;
- g. a termination sequence;
- and
- h. an antibiotic resistance gene.
6 more elements
An isolated nucleic acid molecule operably comprising a plasmid encoding:
- a. a first inducible promoter and a TetO2 sequence;
- b. a Rep78 gene, including a silenced p19 promoter located within the Rep78 gene coding region, wherein the silenced p19 promoter comprises mutations in SP1, TATA-1, and/or TATA-2 sites;
- c. a second inducible promoter and a TetO2 sequence;
- d. a Rep52 gene under control of the second inducible promoter;
- e. termination sequence and;
- f. an antibiotic resistance gene.
3 more elements
An isolated nucleic acid molecule operably comprising:
- a. a first inducible promoter and a TetO2 sequence;
- b. a VP1 gene;
- c. a second inducible promoter and a TetO2 sequence;
- d. a VP2 gene and a VP3 gene;
- and
- e. an antibiotic resistance gene.
3 more elements
Dependencies are inferred from the claim text.