Magnetic hydrogel particles and methods of use
AssigneesGenentech, Inc., The Regents of the University of California
InventorsAlix I CHAN, Christian Nathaniel CUNNINGHAM, Brian PAEGEL, Valerie CAVETT
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Abstract
The present invention relates to particles comprising a magnetic core coated in a polymeric gel, methods of making such particles, and methods of using such particles, for example to support both chemical and biological synthesis.
Claims33
A composition comprising a particle comprising a magnetic core coated in a polymer gel, wherein the polymer gel comprises one or more reagents,
- wherein the one or more reagents comprise a protein, an enzyme, a polynucleotide, oligonucleotide, a polysaccharide, a fluorophore, a lipid, or a supramolecular assembly.
(canceled)
7-8. (canceled)
13-14. (canceled)
(canceled)
A method of producing a composition comprising a particle comprising a magnetic core encapsulated in a polymer gel,
- comprising emulsifying an aqueous solution comprising monomers and magnetic beads with a solution comprising a polymerization initiator to cause polymerization of the monomers and thereby producing a composition comprising a magnetic core encapsulated in the polymer gel;
- wherein a portion of the polymer gel is functionalized with an oligonucleotide, a synthetic hairpin headpiece DNA (HDNA), an alkyne, and/or a primary amine.
(canceled)
A method of producing a composition comprising a particle comprising a magnetic core encapsulated in a polymer gel,
- comprising emulsifying an aqueous solution comprising monomers and magnetic beads with a solution comprising a polymerization initiator to cause polymerization of the monomers and thereby producing a composition comprising a magnetic core encapsulated in the polymer gel;
- wherein the solution comprising monomers and magnetic beads comprises one or more reagents, wherein the one or more reagents comprise a protein, an enzyme, a polynucleotide, an oligonucleotide, a polysaccharide, a fluorophore, or a lipid.
24-40. (canceled)
A method of detecting a synthetic product of a reaction comprising
- synthesizing the synthetic product in a particle comprising a magnetic core coated in a polymer gel, and
- detecting the synthetic product,
- wherein the particle further comprises a template for guiding synthesis of the synthetic product.
42-55. (canceled)
A method of producing a DNA-encoded library comprising incubating a particle comprising a magnetic core coated in an acrylamide gel comprising an oligonucleotide template with a primer that hybridizes to the oligonucleotide template, a DNA polymerase, and oligonucleotides under conditions for DNA synthesis, thereby producing the DNA-encoded library.
A method of producing a DNA-encoded library comprising
- incubating a particle comprising a magnetic core coated in a polyacrylamide gel comprising a functional site and a synthetic hairpin headpiece DNA (HDNA) under conditions that couple a building block to the functional site, and
- incubating the particle with an oligonucleotide comprising a sequence associated with the building block under conditions that couple the oligonucleotide to the HDNA, and thereby producing the DNA-encoded library.
58-64. (canceled)
A method of in vitro translation comprising incubating a particle comprising a magnetic core coated with a polyacrylamide gel comprising a RNA template, ribosomes, and tRNA under conditions for translation of the RNA into a polypeptide.
66-85. (canceled)
Dependencies are inferred from the claim text.