Back to results
US 2026/0092250 A1Published application
Using The Concepts of Metabolic Flux Rate Calculations and Limited Data to Direct Cell Culture Media Optimization and Enable the Creation of Digital Twin Software Platforms
AssigneeMetalytics, Inc.
InventorsEric Jerome Cumming, Samuel Yenne, Daniel Isaac Benjamin, Andy Wiranata Wijaya, Thomas J. Diprospero
Life of this patent
Estimating the term…
Abstract
Provided herein are methods and systems for optimizing media and/or feeding regimes for cells grown in culture by utilizing the analysis of spent cell media in combination with Metabolic Flux Analysis (MFA) and compositions derived from using the same.
Claims26
A method for predicting flux rate of a metabolite of interest in a cell comprising:
- obtaining quantitative measurements or analytical values for components in spent media or cell extracts;
- inputting an indication of the quantitative measurements or the analytical values into a trained neural network, wherein the trained neural network characterizes metabolic flux in the cell based on the quantitative measurements or the analytical values;
- and
- obtaining a predicted flux rate for the metabolite of interest as an output from the trained neural network,
- wherein the output is utilized to characterize how the cell behaves or grows under a first condition.
2 more elements
A system for predicting flux of a metabolite of interest in a cell comprising:
- at least one processor;
- a circuit coupled to the at least one processor and configured to input quantitative measurements or analytical values for components in spent media or cell extracts;
- a circuit configured to characterize the quantitative measurements or analytical values for components in spent media or cell extracts with a trained neural network;
- an input/output (I/O) circuit coupled to the at least one processor;
- a storage circuit coupled to the at least one processor and configured to store data and parameters;
- and
- a memory coupled to the at least one processor comprising computer-readable program code stored in the memory that when executed by the at least one processor causes the at least one processor to perform operations comprising:
- characterizing quantitative measurements or analytical values for components in spent media or cell extracts;
- obtaining a predicted flux rate for a metabolite of interest from characterization of the quantitative measurements or analytical values for components in spent media or cell extracts with the trained neural network;
- and
- controlling output of a determination of a predicted flux rate for the metabolite of interest by way of the I/O circuit,
- wherein the output is utilized to characterize how the cell behaves or grows under a first condition.
9 more elements
A computer-implemented method for predicting flux of a metabolite of interest in a cell comprising:
- inputting quantitative measurements or analytical values for components in spent media or cell extracts into a trained neural network;
- retrieving representative data related to cell metabolism, cellular expansion, cellular proliferation, and/or cellular growth metrics from a storage circuit into the trained neural network;
- characterizing the quantitative measurements or analytical values for components in spent media or cell extracts with the trained neural network, wherein the trained neural network characterizes metabolic flux in the cell based on the quantitative measurements or the analytical values and the representative data related to cell metabolism, cellular expansion, cellular proliferation, and/or cellular growth metrics;
- outputting a predicted flux rate for a metabolite of interest from characterization of the quantitative measurements or analytical values for components in spent media or cell extracts,
- wherein the output is utilized to characterize how the cell behaves or grows under a first condition.
2 more elements
Dependencies are inferred from the claim text.