US 2026/0092087 A1 · Method of Making a Pharmaceutical Composition Comprising a p80 Protein

Abstract

Present invention relates to a method of making a recombinant p80 polypeptide using an E. coli culture comprising a plurality of E. coli host cells each transformed or transfected with an expression vector containing a DNA fragment encoding a recombinant p80 polypeptide; and inducing the E. coli culture by adding a 0.2% L-Arabinose. The isolated, recombinant p80 is added to a pharmaceutical composition, wherein it binds to a therapeutic agent comprising one or more of: drugs, dyes, small molecules, biomolecules, proteins or a combination thereof. The p80 polypeptide enhances the transportation of the therapeutic agent into the bloodstream by the p80 functioning as a tight junction modulator to enhance the permeability of the intestinal epithelium to the therapeutic agent.

Claims9

Dependent claims

A process for the preparation of a recombinant p80 polypeptide, comprising at least the steps of:

a) providing an E. coli culture comprising a plurality of E. coli host cells each transformed with an expression vector containing a DNA fragment encoding a recombinant p80 polypeptide;
b) inducing the E. coli culture by adding a 0.2% L-Arabinose;
c) growing the E. coli culture at 20° C. for 16-18 hr to produce a cell paste;
d) lysing the cell paste;
e) centrifuging the cell paste, and collecting a supernatant from the cell paste;
f) purifying the cell paste using a Ni-NTA affinity column to produce a pool;
g) combining the pool after elution using 250 mM imidazole, and concentrating the pool by using Centriprep-30;
and
h) purifying a plurality of p80 polypeptides from the pool using an anion exchange column.

6 more elements

Dependencies are inferred from the claim text.

Method of Making a Pharmaceutical Composition Comprising a p80 Protein

Life of this patent

Method of Making a Pharmaceutical Composition Comprising a p80 Protein

Life of this patent

Abstract

Claims9