Staining dye-coated cover slip

A method for detecting sperm DNA fragmentation in a semen sample, comprising the steps in the order of:

• (a) embedding the semen sample containing sperm cells in a gel on a carrier slide to obtain a sperm cell-embedding gel immobilized on a carrier slide;
• (b) treating the sperm cells-embedding gel with a lysis solution to lyse the nuclear proteins of the sperm cells embedded in the gel;
• (c) removing liquid from the gel;
• (d) obtaining a cover slip wherein one side of the cover is coated with one or more DNA staining agents, (e) applying an aqueous solution to the gel in step (c) to wet the gel;
• (f) contacting the wet gel with the coated side of the cover slip to stain DNA with the staining agents;
• (g) removing the coverslip from the stained gel;
• (h) rinsing the stained gel to remove unbound staining agents;
• (i) drying the rinsed gel, and (j) examining the dried gel to observe the presence or the absence of a halo formation around a head of each sperm cell to determine SDF.

A method for staining and examining the morphology of a reaction product, comprising the steps in the order of:

• (a) adhering a sample on a carrier slide, wherein the sample is selected from the group consisting of tissues, body fluids, microorganisms, and cells;
• (b) carrying out one or more reactions in the sample on the carrier slide to result in a reaction product on the carrier slide adhering to the carrier slide,
• (c) contacting the reaction product with a coated side of a cover slip for a period of time, wherein the coated side of the cover slip is coated with one or more staining agents, to stain the reaction product with the staining agents;
• (d) placing the carrier slide, the stained reaction product, and the cover slip under microscope to examine the morphology of the stained reaction product.