Psilocybe assay

Abstract

A method for assaying putative Psilocybe spores, Psilocybe tissue, or a cultured host organism that expresses Psilocybe genes comprises obtaining a sample including nucleic acids, contacting the sample with primers for amplifying target nucleic acid sequences, amplifying any of the target nucleic acid sequences when present among the nucleic acids to obtain amplicons, and detecting the amplicons upon amplification of the target nucleic acid sequence. In embodiments, the primers include primers for amplifying (i) a bacterial target nucleic acid sequence, (ii) a Psilocybe target nucleic acid sequence, and/or (iii) a fungal target nucleic acid sequence. For instance, the primers include primers for amplifying a Psilocybecubensis target nucleic acid sequence and a target nucleic acid sequence from at least one other mushroom species subject to misidentification as Psilocybecubensis. A kit may include those primers and probes for detecting the amplicons amplified from any of those target nucleic acid sequences.

Dependencies are inferred from the claim text.