Microsphere formulation for nucleic acid amplification, amplification method, and use in joint detection

Abstract

The present disclosure relates to the technical field of molecular diagnosis, and particularly, to a microsphere formulation for nucleic acid amplification, an amplification method, and use in joint detection. The disclosed microsphere formulation can be stored for a long duration at 2-8° C. When the microsphere preparation is used, no solvent is additionally added, and the microsphere formulation is directly mixed with a sample to be detected. On the premise that the concentration of an original system is not changed, the template content in the system can be remarkably improved, so that the detection sensitivity is improved. By using the microsphere formulation in recombinase polymerase amplification (RPA), recombinase-aided amplification (RAA), or a double or multiple detection formed by combining a second reaction on the basis of RPA or RAA, the sensitivity can be remarkably improved, and meanwhile, the amplification specificity is ensured.

Dependencies are inferred from the claim text.