Pathogen testing device and method

A method for detecting a pathogen in a sample, comprising

• contacting a sample with one or more amplification components in a first region of an assembly, thereby forming a mixture;
• subjecting the mixture to an amplification method;
• and
• contacting the sample with a pH sensor.

A method for detecting a pathogen in a sample, comprising

• (a) contacting a sample with one or more amplification components in a first region of an assembly, thereby forming a mixture;
• (b) subjecting the mixture to an amplification method;
• (c) contacting the sample with a pH sensor;
• and
• (d) identifying whether a detectable signal appears in a second region of the assembly.

A method for detecting a pathogen in a sample, comprising

• (a) contacting a sample with one or more amplification components in a first region of an assembly, thereby forming a mixture;
• (b) subjecting the mixture to an amplification method;
• (c) contacting the sample with a pH sensitive polymer encapsulating an indicator;
• and
• (d) identifying whether a detectable signal appears in a second region of the assembly.

A lateral flow device for detecting a pathogen in a sample, comprising:

• (a) a first region comprising one or more amplification components and a pH sensitive polymer encapsulating an indicator;
• (b) an insulated chamber surrounding the first region;
• (d) a heating element adjacent to the first region;
• and
• (d) a second region comprising an agent that associates with the indicator.

A lateral flow device for detecting a pathogen in a sample, comprising:

• (a) a first region comprising one or more amplification components;
• (b) a pH sensitive polymer encapsulating an indicator;
• (c) an insulated chamber surrounding the first region;
• (d) a heating element adjacent to the first region;
• and
• (e) a second region comprising an agent that associates with the indicator.

A composition comprising:

• (a) one or more amplification components;
• (b) a pH sensitive polymer;
• and
• (c) an indicator.