Karyocreate (karyotype crispr engineered aneuploidy technology)
OwnerTeresa DAVOLI, Nazario BOSCOinventors
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Abstract
Provided are a fusion protein comprising a mutated kinetochore protein and dCas9. The fusion protein is used in conjunction with guide RNAs target the fusion protein to a location of kinetochore assembly on a centromere such that the fusion protein interferes with chromosome segregation. Use of the fusion protein and the guide RNAs in cells results the cells acquiring an aneuploidy karyotype. Expression vectors that encode the fusion proteins and/or the guide RNAs and their uses in the method of producing an aneuploidy karyotype are also provided.
Claims16
A fusion protein comprising a mutated kinetochore protein and dCas9.
A method comprising selecting a guide RNA that targets a location of kinetochore assembly on a centromere of a specific chromosome, and introducing into cells a combination of the selected guide RNA and a fusion protein comprising a mutated kinetochore protein and dCas9, and allowing cell divisional in the presence of the selected guide RNA and the fusion protein such that divided cells comprise an aneuploidy karyotype.
Dependencies are inferred from the claim text.