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AssigneeConfluence Genetics, LLC
InventorsMatthew Brett Begemann, Emma Elizabeth January, Gina C. Neumann, Allison Jane Newton Antonakos, Anna Singer, Erin Zess
Estimating the term…
Compositions and methods for targeting pre-determined DNA sequences in cells of interest are provided. The methods result in the targeted elimination of cells that comprise the pre-determined DNA sequence(s). Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cas12a2 protein operably linked to a promoter that is operable in the cells of interest. Methods to use these DNA constructs to selectively target and eliminate cells that harbor the targeted DNA sequence(s) are described herein.
A composition comprising a Cas12a2 polypeptide or a polynucleotide encoding the Cas12a2 polypeptide, wherein the Cas12a2 polypeptide shares at least 85% identity with a sequence selected from the group consisting of SEQ ID NOs:
A method for binding, cleaving, and/or modifying a target sequence in one or more cells of interest comprising delivering to said one or more cells
A cell, a plant, a plant part, or a plant pathogen comprising a composition comprising a Cas12a2 polypeptide or a polynucleotide encoding the Cas12a2 polypeptide, wherein the Cas12a2 polypeptide shares at least 85% identity with a sequence selected from the group consisting of SEQ ID NOs:
Dependencies are inferred from the claim text.