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US 2026/0085292 A1Published application
Minimal essential media for the culture of human induced pluripotent stem cells
AssigneeNorthwestern University
InventorPaul Wesley Burridge
Life of this patent
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Abstract
Disclosed is minimal essential media for culturing induced human pluripotent stem cells (iPSCs), methods for preparing the media, and methods of using the media.
Claims25
A media for culturing induced human pluripotent stem cells (iPSCs), wherein the media comprises components for culturing iPSCs, wherein the components comprise
- a) a salt solution, comprising Calcium chloride (CaCl2)), Iron(II) sulfate heptahydrate (FeSO4·7H2O), Magnesium sulfate (MgSO4), Potassium chloride (KCl), Sodium bicarbonate (NaHCO3), Sodium chloride (NaCl), Sodium phosphate monobasic monohydrate (NaH2PO4·H2O) and Zinc sulfate heptahydrate (ZnSO4·7H2O);
- b) a vitamin solution comprising Biotin (B7), Choline bitartrate, D-Pantothenic acid hemicalcium salt (B5), Folic acid (B9), Nicotinamide (B3), Pyridoxine hydrochloride (B6), Riboflavin (B2), and myo-Inositol;
- and
- c) an amino acid solution comprising Glycine, L-Arginine HCl, L-Cysteine HCl·H2O, L-Glutamine, L-Histidine HCl·H2O, L-Isoleucine, L-Leucine, L-Lysine HCl, L-Methionine, L-Phenylalanine, L-Threonine, L-Tryptophan, L-Tyrosine disodium salt hydrate, and L-Valine.
1 more element
Dependencies are inferred from the claim text.