Stem cell-derived gastruloid model, method for constructing same, and use

A method for constructing a gastruloid model, wherein, the method comprises the following steps:

• (1) digesting pluripotent stem cells into single cells when the cells grow to 80%-90% confluence, and resuspending the cells using PBS to obtain a cell suspension 1;
• taking the cell suspension 1 and adding a GIER culture medium containing Y-27632 to further resuspend the cells to obtain a cell suspension 2;
• inoculating the cell suspension 2 into an embryoid body culture plate coated with an anti-adhesion solution in advance, and centrifuging;
• and
• replacing half of the culture medium with a GIER culture medium on the second day of culture, and replacing half of the culture medium every day until a primitive endoderm and a primitive ectoderm are formed;
• (2) discarding ½ to ¾ of the volume of the GIER culture medium in each well of the culture plate in step (1) and adding a G2EE culture medium for culturing, and performing amniotic cavity pre-induction;
• (3) discarding ½ to ¾ of the volume of the G2EE medium in each well of the culture plate in step (2) and adding an aG2EE medium for culturing, and inducing the formation of amnion cells;
• and
• (4) discarding ½ to ¾ of the volume of the aG2EE culture medium in each well of the culture plate in step (3) and adding a bG2EE culture medium for culture until the cells are induced into amnion cells, primitive streak cells, and primordial germ cells, the post-implantation gastruloid model is obtained.