High-speed dispensing spotter for biological sample and cell dispensing method of high-speed dispensing spotter

A nozzle tip of a high-speed dispensing spotter, which is detachably connectable to a nozzle of a head part of a high-speed dispensing spotter and made of an elastic material, the nozzle tip comprising:

• an inlet into which the nozzle is inserted and connected;
• a ring-shaped protrusion extending from the inlet and protruding from an inner surface thereof to prevent the nozzle from entering a body part of the nozzle;
• and
• a body part extending from the protrusion and having a funnel-shaped discharge part from which a biological sample inside the body part is discharged by air pressure.

A method for counting the number of dispensing events using a high-speed dispensing spotter comprising:

• (a) discharging a sample from a discharge part of a nozzle tip through a nozzle of the high-speed dispensing spotter;
• (b) dispensing the discharged sample onto a well or a pillar through an opening of a drop counter module;
• and
• (c) counting the number of dispensing events of the sample by the drop counter module by detecting passage of the sample through the opening of the drop counter module.

A drop counter module, which is coupled to a lower portion of a nozzle tip connected to a nozzle of a high-speed dispensing spotter, the drop counter module comprising:

• an opening through which a sample discharged from the nozzle tip passes;
• an LED emitting portion which emits infrared light or visible light across the opening;
• and
• an LED receiving portion which receives the infrared light or visible light from the LED emitting portion,
• wherein the drop counter module is configured to count the number of dispensing events of the sample by detecting the passage of the sample through the opening.