Polymeric multilayer film for use in bioprocessing applications

A flexible bioprocessing container comprising at least a polymeric, multilayer film comprising:

• a sealant layer comprising:
• (i) 58-95.5 wt % in respect of the total weight of the sealant layer of one or more ethylene/alpha olefin copolymers having an average density lower than 0.922 g/cc;
• (ii) 4-10 wt % in respect of the total weight of the sealant layer of one or more polymers having an average density higher than or equal to 0.950 g/cc and selected among one or more ethylene/alpha olefin copolymers and/or one or more ethylene homopolymers;
• (iii) 0.5-2.0 wt % in respect of the total weight of the sealant layer of high molecular weight siloxane polymers;
• an outer skin layer comprising one or more polymers selected from the group consisting of:
• (co)polyesters, polyamides;
• high-density polyethylene (HDPE), polypropylene (PP) and their admixtures,
• wherein said film is sealed to define an interior compartment of the container and wherein the sealant layer of said film forms an inner surface of the container.

A method for culturing cells comprising either the steps of:

• (i) providing a flexible bioprocessing container;
• (ii) introducing a liquid medium into the interior compartment of the container;
• (iii) inoculating the liquid medium with cells, and
• (iv) incubating the cells within the interior compartment of the container under suitable conditions for cell growth;
• or the steps of:
• (i) providing a flexible bioprocessing container;
• (ii) pre-inoculating a liquid medium with cells;
• (iii) introducing the pre-inoculated liquid medium into the interior compartment of the container, and
• (iv) incubating the cells within the interior compartment of the container under suitable conditions for cell growth;
• in either instance, the flexible bioprocessing container comprising at least a polymeric, multilayer film comprising:
• a sealant layer comprising:
• (i) 58-95.5 wt % in respect of the total weight of the sealant layer of one or more ethylene/alpha olefin copolymers having an average density lower than 0.922 g/cc;
• (ii) 4-10 wt % in respect of the total weight of the sealant layer of one or more polymers having an average density higher than or equal to 0.950 g/cc and selected among one or more ethylene/alpha olefin copolymers and/or one or more ethylene homopolymers;
• (iii) 0.5-2.0 wt % in respect of the total weight of the sealant layer of high molecular weight siloxane polymers;
• an outer skin layer comprising one or more polymers selected from the group consisting of:
• (co)polyesters, polyamides;
• high-density polyethylene (HDPE), polypropylene (PP) and their admixtures.