Alpha-amylase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsCarsten ANDERSEN, Chakshusmathi Ghadiyaram, Rajendra Kulothungan Sainathan, Padmavathi Balumuri, Padma Venkatachalam Iyer, Iben Damager, Astrid Munch, Sohel Dalal
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Abstract
The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims33
A variant of a parent polypeptide having alpha-amylase activity, wherein said variant is a fusion polypeptide which has an improved wash performance at low temperature, and wherein said variant has alpha-amylase activity.
A method of improving wash performance of a parent polypeptide having the amino acid sequence of SEQ ID NO:
- 13 or 14, or having at least 80% sequence identity thereto, said method comprising the steps of:
- a) a substitution and/or deletion of two, three or four positions in the parent alpha-amylase said positions corresponding to positions 181, G182, D183, and G184 of the mature polypeptide of SEQ ID NO:
- 4;
- and
- b) an alteration at one or more positions corresponding to positions H1, T5, G7, Q11, N16, V17, Q32, A37, T40, P45, W48, G50, T51, N54, V56, K72, R87, Q98, M105, G109, F113, R116, Q118, Q125, G133, T134, W140, G142, G149, T165, W167, Q169, R171, Q172, L173, A174, G184, A186, E190, T193, N195, A204, V206, P211, I214, V215, L217, L219, A225, L235, V238, K242, S244, M246, M248, L250, G255, Q256, N260, A263, V264, Y267, K269, N270, G273, S280, W284, T285, M286, F289, V291, N295, Q299, R320, H321, S323, H324, V326, F328, T334, D337, Q345, G346, G348, T355, S376, D377, D379, Y382, S383, Q385, K391, K393, Q395, A420, G423, T444, A445, Q449, T459, P473, C474, G476, G477, and K484 of SEQ ID NO:
- 13;
- or H1, A37, T40, N54, V56, A60, K72, G109, F113, R116, N125, F133, T134, T165, Q169, Q172, L173, A174, G184, A186, E190, N195, G196, A204, V206, A225, L228, K242, S244, G255, N260, A265, K269, S280, W284, N295, S304, R320, H321, S323, V326, K391, 1405, A421, A422, A428, G448, D476, and G477 of SEQ ID NO:
- 14,
- wherein the variant has at least 80% but less than 100% sequence identity with the polypeptide of SEQ ID NO:
- 4 or 7, and wherein said variant has alpha-amylase activity and improved wash performance compared to said parent polypeptide.
(canceled)
Dependencies are inferred from the claim text.