Methods of antigen production
AssigneesThe Wistar Institute of Anatomy and Biology, Inovio Pharmaceuticals, Inc., Geneos Therapeutics, Inc.
InventorsElizabeth Kennedy Duperret, Alfredo Perales Puchalt, David B. Weiner, Niranjan Y. Sardesai
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Abstract
The disclosure relates to methods of manufacturing individualized vaccines that comprise nucleic acid molecules that encode one or more neoantigens specific for antigens that are expressed by a tumor in a subject. Compositions comprising coding regions encoding neoantigens organized in a pattern of nucleic acid sequences are also disclosed as well as methods of immunizing a subject using the same.
Claims20
A method of making a therapeutic composition for a subject diagnosed with a cancer, the method comprising:
- analyzing a sample from the subject to identify a plurality of neoantigen mutations;
- producing, based on the plurality of neoantigen mutations, an immunogenic composition;
- contacting isolated dendritic cells from the subject with the immunogenic composition;
- administering to the subject the dendritic cells;
- isolating a population of CD8+ T cells from a peripheral blood sample from the subject that recognize at least one of the plurality of neoantigen mutations;
- and
- expanding the population of CD8+ T cells that recognize the at least one of the plurality of neoantigen mutations.
The method of claim further comprising adding to the therapeutic composition one or more combination therapeutics.
Dependencies are inferred from the claim text.