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US 2026/0083845 A1Published application
Engineered immune cells
AssigneeCellicure, Inc.
InventorsMinggang Zhang, Belle X. Wang, Yongmei Li, Nathan X. Li, Qisheng Li
Life of this patent
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Abstract
The present application relates to chimeric antigen receptors (CARs), engineered immune cells (e.g., NK cells, NKT cells, and T cells), and methods of use thereof. The CARs provided herein can enhance the killing activities of the engineered NK cells.
Claims31
A chimeric antigen receptor (CAR), comprising:
- (i) an extracellular antigen binding domain;
- (ii) a transmembrane domain;
- and
- (iii) an intracellular signaling domain comprising an intracellular domain derived from a first polypeptide,
- wherein the intracellular domain derived from the first polypeptide is linked to the C-terminus of the transmembrane domain directly or via a peptide linker, and
- wherein the first polypeptide is selected from:
- DAP10, DAP12 and DNAM1;
- wherein the intracellular signaling domain of the CAR further comprises an intracellular domain derived from CD3zeta.
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The CAR of 12, from N-terminus to C-terminus, comprising:
- (i) an extracellular antigen binding domain;
- (ii) a transmembrane domain comprising an transmembrane domain derived from the second polypeptide;
- (iii) an intracellular signaling domain sequentially comprising
- (iii)-(a) an intracellular domain derived from DAP10;
- (iii)-(b) an intracellular domain derived from CD3zeta;
- and
- (iii)-(c) an intracellular domain derived from SAP or an intracellular domain derived from EAT2,
- wherein the intracellular domains within the intracellular signaling domain are linked to each other directly or via one or more peptide linkers, and
- wherein the extracellular antigen binding domain, the transmembrane domain, and the intracellular domain are linked to each other directly or via one or more peptide linkers.
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Dependencies are inferred from the claim text.