Device for parallel detection and quantification of nucleic acid based markers
AssigneeToDx Inc.
InventorsNoam Kedem Mamet, Gil Harari
Life of this patent
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Abstract
The present disclosure describes methods and devices for detection and quantification of nucleic acid based markers in a sample.
Claims35
A device comprising a first external surface, a second external surface that is different from the first external surface, an internal surface, a plurality of targeting nucleic acids immobilized to pre-determined locations on the internal surface, and a reaction chamber enclosed by the internal surface, wherein targeting nucleic acids specific for different target polynucleotides are immobilized at different predetermined locations on the internal surface.
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The device of claim wherein (1) the first reagent chamber contains reaction buffer, and the second reagent chamber contains dried enzymes, and wherein the reaction buffer dispenses into the second reagent chamber to dissolve the dried enzymes to form a reaction mix which then dispenses into the reaction chamber;
- (2) the second reagent chamber is disposed downstream of the first reagent chamber and upstream of the reaction chamber;
- (3) the first reagent chamber, the second reagent chamber, and the reaction chamber are horizontally aligned;
- and/or (4) the second valve controlling fluid flow from the reagent compartment to the reaction chamber controls the fluid flow from the second reagent chamber to the reaction chamber.
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Dependencies are inferred from the claim text.