METHODS FOR TREATING OR PREVENTING RESPIRATORY DISEASES IN CATTLE USING POLYMERS AND POLYPLEXES FOR mRNA DELIVERY AND mRNA THERAPY

A method of preventing or treating or controlling a respiratory disease in a bovine comprising:

• delivering to a lung or a trachea of the bovine a pharmaceutical composition comprising a pharmaceutically acceptable carrier and a polyplex,
• wherein the polyplex comprises:
• one or more, optionally further modified, mRNA encoded with a molecule selected from the group consisting of glycosylphosphatidylinositol (GPI) complex, antibody immunoglobulin G (IgG), antibody immunoglobulin A (IgA), antibody immunoglobulin M (IgM), antimicrobial peptide Bactenecin 5 (Bac5), antimicrobial peptide Bactenecin 7 (Bac7), bovine myeloid antimicrobial peptide (BMAP-28), lipoxin inducing enzymes, resolvin inducing enzymes, cytokines, CRISPR associated protein 13 (Cas13) enzymes, nanoluciferase (NLuc) proteins, and gene activator, catalytically dead CRISPR-associated protein 9 fused to an activator selected from the group consisting of VP64-p65-Rta transactivation domain (dCas9-VPR), VP64, and VP64-p65-HSF1 on the N terminus and SS18 on the C-terminus,
• a polymer prepared by
• a. polymerizing components i-ii and optionally iii:
• i. a diacrylate monomer;
• ii. one or more linker monomers including one or more functional groups independently selected from the group consisting of amino groups, alcohol groups and thiol groups;
• and
• iii. one or more branching monomers including one or more functional groups independently selected from the group consisting of amino groups, alcohol groups and thiol groups;
• and
• b. end-capping the product of step (a) with one or more end capping monomers including one or more functional groups independently selected from the group consisting of amino groups and alcohol groups.