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US 2026/0076998 A1Published application
Method for obtaining an umbilical cord extract
AssigneeThe Universidad San Francisco De Quito Usfq
InventorsAndres Bernardo CAICEDO PALIZ, Michelle Amanda GRUNAUER ANDRADE, Luis Alfonso EGUIGUREN LEÓN, Radmila Francesca VELARDE
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Abstract
The present invention relates to a method for obtaining an umbilical cord extract that can be used for tissue regeneration or repair, for medical or cosmetic uses, as a preparation base for liquid, semisolid or solid grafts or dressings, binders or polymerising agents, or in the subsequent purification of the regenerative components thereof.
Claims13
A method for preparing an umbilical cord extract comprising the steps of:
- a—reserving between one and five umbilical cords after birth in a physiological or buffered solution;
- b—wash, fractionate and homogenize the umbilical cords in a buffered cell culture medium;
- and
- c—centrifuge the mixture obtained in b-and filter a crude liquid fraction obtained with a clarification filter between 1 μm to 100 μm, followed by a second filtration by pore size for microfiltration between 0.1 μm to 1 μm, where all the steps of the method are carried out at a temperature between 1 and 10° C.
1 more element
An umbilical cord extract obtained using the method of:
- reserving between one and five umbilical cords after birth in a physiological or buffered solution;
- b—wash, fractionate and homogenize the cords in a buffered cell culture medium;
- and
- c—centrifuge the mixture obtained in b—and filter the crude liquid fraction obtained with a clarification filter between 1 μm to 100 μm, followed by a second filtration by pore size for microfiltration between 0.1 μm to 1 μm,
- wherein all the steps of the method are carried out at a temperature between 1 and 10° C.
2 more elements
Dependencies are inferred from the claim text.