Density-based immunophenotyping

A system for determining an immunophenotype of a tumor, comprising:

• a computing system comprising
• one or more non-transitory computer-readable storage media storing computer program instructions;
• and
• one or more processors coupled to the one or more non-transitory computer-readable storage media, the one or more processors being configured to execute the computer program instructions to:
• receive an image of a tumor region;
• divide the image into a plurality of tiles each depicting at least one of tumor epithelium or tumor stroma;
• for each of the plurality of tiles:
• calculate an epithelium-immune cell density of the tile based on a number of immune cells identified in the tumor epithelium or calculate a stroma-immune cell density of the tile based on a number of immune cells identified in the tumor stroma;
• and
• determine, based on the stroma-immune cell density and/or the epithelium-immune cell density, an inflammation type of the tile as being a first inflammation type or a second inflammation type;
• and
• determine a tumor immunophenotype for the image based on the inflammation type of the plurality of tiles.

A non-transitory computer-readable medium comprising computer program instructions that, when executed by one or more processors of a computing system, effectuate operations comprising:

• receiving an image of a tumor region;
• dividing the image into a plurality of tiles each depicting at least one of tumor epithelium or tumor stroma;
• for each of the plurality of tiles:
• calculating an epithelium-immune cell density of the tile based on a number of immune cells identified in the tumor epithelium or calculating a stroma-immune cell density of the tile based on a number of immune cells identified in the tumor stroma;
• and
• determining, based on the stroma-immune cell density and/or the epithelium-immune cell density, an inflammation type of the tile as being a first inflammation type or a second inflammation type;
• and
• determine a tumor immunophenotype for the image based on the inflammation type of the plurality of tiles.