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AssigneeJiangnan University
InventorsZhen KANG, Jian CHEN, Ruirui XU, Guocheng DU, Jianghua LI, Yang WANG
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Disclosed is a method for constructing a synthesis and regeneration system based on APS as an active sulfonate donor, belonging to the technical field of biology. The present disclosure provides a new purpose of APS as an active sulfonate donor, and greatly improves the synthesis efficiency of the APS by screening different ATP sulfurylases and adding a pyrophosphatase into a reaction system to eliminate pyrophosphate as a byproduct. Further, the construction of a sulfonation modification system is realized by constructing an APS circulation regeneration system. Compared with a PAPS regeneration system, the APS circulation regeneration system has the advantages of short path and high efficiency, the sulfonation modification efficiency is significantly improved, and the synthesis cost is successfully reduced.
A 3′-phosphoadenosine-5′-phosphosulfate (PAPS) regeneration circulation system, comprising a polyphosphate kinase, a 3′-adenosine phosphate-5′-phosphate (PAP) dephosphorylation enzyme, and a PAPS synthesis bifunctional enzyme which is a combination of an ATP sulfurylase and an APS kinase, being applicable to PAPS synthesis through catalysis by one or more of the above enzymes from any one substance of AMP, ADP, ATP, APS, or PAP and wherein the PAPS regeneration circulation system comprises catalyzing module I, module II, and module III forming a closed-loop cycle, wherein the catalyzing module I converts the AMP into the ATP, the catalyzing module II converts the ATP into the PAPS, and the catalyzing module III converts the PAPS into the AMP.
Dependencies are inferred from the claim text.