Method for a preparation of a molecularly-imprinted-polymer (MIP) electrochemical sensor for an electrochemically inactive analyte detection, a method for using said sensor and such a sensor

The method according to claim 7, comprising the steps wherein:

• step (1) comprises formation of a cleavable linking layer on the electrode,
• by a formation of a 3-(2-pyridyldithio) propionyl hydrazide (PDPH) monolayer on the redox-active surface (RuO2) of the electrode;
• and
• by a formation of a 3,3′-dithiobis(sulfosuccinimidyl propionate) (DTSSP) monolayer covalently linked to the 3-(2-pyridyldithio) propionyl hydrazide (PDPH) monolayer;
• step (2) comprises immobilization of an electochemically inactive protein molecules on the cleavable linking layer;
• step (3) comprises polymerization of m-phenylenediamine (m-PD) on the electochemically inactive protein molecules immobilized onto the electrode thereby forming a polymeric layer coating on said electrically conductive surface with entrapped electochemically inactive protein molecules;
• and
• step (4) comprises cleavage of the cleavable linking layer thereby removing the electochemically inactive protein molecules from the polymeric layer and obtaining the MIP layer.

A method for using a MIP electrochemical sensor characterized by comprising the steps of:

• contacting the electrochemical sensor with a sample solution comprising a target analyte for a defined time period, wherein the electrochemical sensor comprises at least one working electrode, a reference electrode and a counter electrode integrated on the insulative substrate, wherein a working electrode comprises a redox-active surface, wherein the redox-active surface is coated by a molecular imprinted polymer (MIP) layer, and wherein the target analyte selectively binds to a plurality of analyte-selective cavities within the MIP layer;
• imposing on the working electrode an electrochemical potential with the aid of a potentiostat;
• and
• measuring an electrochemical current with the aid of a potentiostat, wherein the electrochemical current is calibrated to a concentration of the target analyte in the sample solution.