Detection and digital quantitation of multiple targets

A method for performing a digital assay, the method comprising:

• (a) obtaining a sample comprising a plurality of target nucleic acid molecules;
• (b) generating a plurality of partitions, wherein said plurality of partitions comprises at least 1 million partitions, wherein a partition of the plurality of partitions comprises:
• (i) materials for an amplification reaction, wherein the materials for the amplification reaction comprise primers comprising locked nucleic acid (LNA);
• and
• (ii) less than or equal to one target nucleic acid molecule of the plurality of target nucleic acid molecules;
• (c) amplifying said plurality of target nucleic acid molecules within the plurality of partitions;
• and
• (d) detecting signals from said plurality of partitions, thereby generating counts corresponding to the plurality of target nucleic acid molecules.

A method for performing a digital assay, the method comprising:

• (a) obtaining a sample comprising a plurality of target nucleic acid molecules associated with minimal residual disease (MRD) detection;
• (b) generating a plurality of partitions, wherein said plurality of partitions comprises at least 1 million partitions, wherein a partition of the plurality of partitions comprises:
• (i) materials for an amplification reaction;
• and
• (ii) less than or equal to one target nucleic acid molecule of the plurality of target nucleic acid molecules;
• (c) amplifying said plurality of target nucleic acid molecules within the plurality of partitions;
• and
• (d) detecting signals from said plurality of partitions, thereby generating counts corresponding to the plurality of target nucleic acid molecules.

A method for performing a digital assay, the method comprising:

• (a) obtaining a sample comprising a plurality of target nucleic acid molecules, wherein the plurality of target nucleic molecules comprises single nucleotide polymorphism (SNP) targets;
• (b) generating a plurality of partitions, wherein said plurality of partitions comprises at least 1 million partitions, wherein a partition of the plurality of partitions comprises:
• (i) materials for an amplification reaction, wherein the materials comprise a primer comprising locked nucleic acid (LNA);
• and
• (ii) less than or equal to one target nucleic acid molecule of the plurality of target nucleic acid molecules;
• (c) amplifying said plurality of target nucleic acid molecules within the plurality of partitions;
• and
• (d) detecting signals from said plurality of partitions, thereby generating counts corresponding to the plurality of target nucleic acid molecules.